Electron transfer between guanosine radical and amino acids in aqueous solution. 1. Reduction of guanosine radical by tyrosine Научная публикация
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The Journal of Physical Chemistry B
ISSN: 1520-6106 |
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| Вых. Данные | Год: 2007, Том: 111, Номер: 25, Страницы: 7439-7448 Страниц : 10 DOI: 10.1021/Jp067722i | ||||||||
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Реферат:
As a model of chemical DNA repair, the reductive electron transfer from the aromatic amino acid tyrosine to the radical of the purine base guanosine monophosphate (GMP) was studied by time-resolved chemically induced dynamic nuclear polarization (CIDNP). The guanosyl radicals were photochemically generated in the quenching reaction of the triplet excited dye 2,2'-dipyridyl. Depending on the pH of the aqueous solution, four different guanosyl radicals were observed. The identification of the radicals was possible because of the high sensitivity of CIDNP to distinguish them through their ability or disability of participating in the degenerate electron hopping reaction with the diamagnetic molecules of guanosine monophosphate in the ground state. The CIDNP kinetics in this three-component system containing the dye, GMP, and N-acetyl tyrosine is strongly dependent on the efficiency of the electron-transfer reaction from tyrosine to the nucleotide radical. Quantitative analysis of the CIDNP kinetics obtained at different concentrations of the amino acid, together with the comparison with the CIDNP kinetics of the two-component systems (dipyridyl/tyrosine and dipyridyl/GMP) allowed for the determination of the rate constant k(e) of the reductive electron-transfer reaction for five pairs of reactants, with different protonation states depending on the pH: GH(++center dot)/TyrOH (pH 1.3), G(+center dot)/TyrOH (pH 2.9), G(-H)(center dot)/TyrOH (pH 7.5), G(-H)(center dot)/TyrO(-) (pH 11.3), and G(-2H)(-center dot)/TyrO(-) (pH 13.3). The rate constant k(e) varies from (7.1 +/- 3.0) x 10(8) M-1 s(-1) (pH 1.3, 2.9) to less than 6 x 10(6) M-1 s(-1) (pH 13.3).
Библиографическая ссылка:
Morozova O.B.
, Kiryutin A.S.
, Sagdeev R.Z.
, Yurkovskaya A.V.
Electron transfer between guanosine radical and amino acids in aqueous solution. 1. Reduction of guanosine radical by tyrosine
The Journal of Physical Chemistry B. 2007. V.111. N25. P.7439-7448. DOI: 10.1021/Jp067722i WOS OpenAlex
Electron transfer between guanosine radical and amino acids in aqueous solution. 1. Reduction of guanosine radical by tyrosine
The Journal of Physical Chemistry B. 2007. V.111. N25. P.7439-7448. DOI: 10.1021/Jp067722i WOS OpenAlex
Даты:
| Поступила в редакцию: | 21 нояб. 2006 г. |
| Опубликована online: | 25 мая 2007 г. |
| Опубликована в печати: | 1 июн. 2007 г. |
Идентификаторы БД:
| Web of science: | WOS:000247435700063 |
| OpenAlex: | W2001458948 |