Abstract: This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine, lactate, phosphocholine and reduced glutathione. The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan, tyrosine, carnitine, glycerophosphate, GSH and GSSG were found in lenses of senescence-accelerated OXYS rats; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress.

Cite: Yanshole V.V. , Snytnikova O.A. , Kiryutin A.S. , Yanshole L.V. , Sagdeev R.Z. , Tsentalovich Y.P.
Metabolomics of the rat lens: A combined LC-MS and NMR study
Experimental Eye Research. 2014. Т.125. С.71-78. DOI: 10.1016/j.exer.2014.05.016 WOS OpenAlex

Dates:

Submitted:	Mar 20, 2014
Accepted:	May 27, 2014
Published online:	Jun 5, 2014
Published print:	Aug 1, 2014

Identifiers:

Web of science:	WOS:000340079300008
OpenAlex:	W2167490956

Citing:

DB	Citing
Web of science	51
OpenAlex	55

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