Metabolomic composition of normal aged and cataractous human lenses | Sciact

Metabolomic composition of normal aged and cataractous human lenses Научная публикация

Журнал

Experimental Eye Research
ISSN: 0014-4835

Вых. Данные

Год: 2015, Том: 134, Страницы: 15-23 Страниц : 9 DOI: 10.1016/j.exer.2015.03.008

Ключевые слова

Metabolomics, Lens, Cataract, Antioxidants, UV filters, Aging

Авторы

Tsentalovich Yuri P. ^1,2 , Verkhovod Timofey D. ¹ , Yanshole Vadim V. ^1,2 , Kiryutin Alexey S. ¹ , Yanshole Lyudmila V. ^1,2 , Fursova Anjella Zh. ³ , Stepakov Denis A. ⁴ , Novoselov Vladimir P. ⁴ , Sagdeev Renad Z. ^1,2

Организации

1	International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia
2	Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia
3	Novosibirsk State Regional Clinical Hospital, Nemirovicha-Danchenko 130, Novosibirsk 630087, Russia
4	Novosibirsk Regional Clinical Bureau of Forensic Medical Examination, Nemirovicha-Danchenko 134, Novosibirsk 630087, Russia

Quantitative metabolomic profiles of normal and cataractous human lenses were obtained with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. The concentration of more than fifty metabolites in the lens cortex and nucleus has been determined. For the majority of metabolites, their concentrations in the lens cortex and nucleus are similar, which confirms low metabolic activity in the lens core. The difference between the metabolite levels in the cortex and nucleus of the normal lens is observed for antioxidants and UV filters, which demonstrates the activity of redox processes in the lens. A huge difference is found between the metabolomic compositions of normal and age-matched cataractous lenses: the concentrations of almost all metabolites in the normal lens are higher than in the cataractous one. The most pronounced difference is observed for compounds playing a key role in the lens cell protection and metabolic activity, including antioxidants, UV filters, and osmolytes. The results obtained imply that the development of the age-related cataracts might originate from the metabolic dysfunction of the lens epithelial cells.

Tsentalovich Y.P. , Verkhovod T.D. , Yanshole V.V. , Kiryutin A.S. , Yanshole L.V. , Fursova A.Z. , Stepakov D.A. , Novoselov V.P. , Sagdeev R.Z.
Metabolomic composition of normal aged and cataractous human lenses
Experimental Eye Research. 2015. V.134. P.15-23. DOI: 10.1016/j.exer.2015.03.008 OpenAlex

Поступила в редакцию:	3 февр. 2015 г.
Принята к публикации:	11 мар. 2015 г.
Опубликована online:	12 мар. 2015 г.

OpenAlex:

W2024293221

БД	Цитирований
OpenAlex	75