Abstract: Parahydrogen-induced polarization (PHIP) is a source of nuclear spin hyperpolarization, and this technique allows for the preparation of biomolecules for in vivo metabolic imaging. PHIP delivers hyperpolarization in the form of proton singlet order to a molecule, but most applications require that a heteronuclear (e.g. 13C or 15N) spin in the molecule is hyperpolarized. Here we present high field pulse methods to manipulate proton singlet order in the [1-13C]fumarate, and in particular to transfer the proton singlet order into 13C magnetization. We exploit adiabatic pulses, i.e., pulses with slowly ramped amplitude, and use constant-adiabaticity variants: the spin Hamiltonian is varied in such a way that the generalized adiabaticity parameter is time-independent. This allows for faster polarization transfer, and we achieve 96.2% transfer efficiency in thermal equilibrium experiments. We demonstrate this in experiments using hyperpolarization, and obtain 6.8% 13C polarization. This work paves the way for efficient hyperpolarization of nuclear spins in a variety of biomolecules, since the high-field pulse sequences allow individual spins to be addressed.

Cite: Rodin B.A. , Kozinenko V.P. , Kiryutin A.S. , Yurkovskaya A. , Eills J. , Ivanov K.L.
Constant-adiabaticity pulse schemes for manipulating singlet order in 3-spin systems with weak magnetic non-equivalence
Journal of Magnetic Resonance. 2021. V.327. P.106978. DOI: 10.1016/j.jmr.2021.106978 WOS OpenAlex

Dates:

Submitted:	Oct 20, 2020
Accepted:	Apr 1, 2021
Published online:	Apr 5, 2021

Identifiers:

Web of science:	WOS:000652024400013
OpenAlex:	W3147461800

Citing:

DB	Citing
OpenAlex	21

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