Abstract: Two-dimensional (2D) NMR is one of the most important spectroscopic tools for the investigation of biological macromolecules. However, owing to the low sensitivity of NMR it takes usually from several minutes to many hours to record such a spectrum. Here we show that a bioactive derivative of the sunflower trypsin inhibitor-1 (SFTI-1), a tetradecapeptide, can be detected by the combination of parahydrogen-induced polarization (PHIP) and ultrafast 2D-NMR spectroscopy (in the following abbreviated as 2D-NMR). The PHIP activity of the inhibitor was achieved by labeling with O-propargyl-L-tyrosine. In 1D-PHIP experiments an enhancement of approximately 1200 compared to normal NMR was found. This enhancement permits measurement of 2D-NMR correlation spectra of low concentrated SFTI-1 in less than 10 seconds, employing ultrafast single-scan 2D-NMR detection. As experimental examples PHIP assisted ultrafast single-scan TOCSY spectra of SFTI-1 are shown.

Cite: Kiryutin A.S. , Sauer G. , Tietze D. , Brodrecht M. , Knecht S. , Yurkovskaya A.V. , Ivanov K.L. , Avrutina O. , Kolmar H. , Buntkowsky G.
Ultrafast Single-Scan 2D-NMR Spectroscopic Detection of a PHIP-Hyperpolarized Protease Inhibitor
Chemistry - A European Journal. 2019. V.25. N16. P.4025-4030. DOI: 10.1002/chem.201900079

Dates:

Submitted:	Jan 4, 2019
Accepted:	Jan 30, 2019
Published online:	Jan 30, 2019
Published print:	Feb 17, 2019

Identifiers: No identifiers

Citing: Пока нет цитирований

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