PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI

PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI Научная публикация

Журнал

Scientific Reports
ISSN: 2045-2322

Вых. Данные

Год: 2021, Том: 11, Номер: 1, Номер статьи : 5646, Страниц : 12 DOI: 10.1038/s41598-021-85136-2

Авторы

Svyatova Alexandra ^1,2,3 , Kozinenko Vitaly P. ^1,2 , Chukanov Nikita V. ^1,2 , Burueva Dudari B. ^1,2 , Chekmenev Eduard Y. ^4,5,6,7 , Chen Yu-Wen ⁸ , Hwang Dennis W. ⁸ , Kovtunov Kirill V. ^1,2 , Koptyug Igor V. ¹

Организации

1	International Tomography Center SB RAS, 3A Institutskaya St., Novosibirsk, Russia, 630090
2	Novosibirsk State University, 2 Pirogova St., Novosibirsk, Russia, 630090
3	Institute of Cytology and Genetics SB RAS, 10 Ac. Lavrentieva Ave., Novosibirsk, Russia, 630090
4	Department of Chemistry, Wayne State University, Detroit, MI, 48201, USA
5	Integrative Biosciences, Wayne State University, Detroit, MI, 48201, USA
6	Karmanos Cancer Institute, Wayne State University, Detroit, MI, 48201, USA
7	Russian Academy of Sciences, Moscow, Russia, 119991
8	Institute of Biomedical Sciences, Academia Sinica, Taipei, 115, Taiwan (Republic of China)

Parahydrogen-induced polarization of 13C nuclei by side-arm hydrogenation (PHIP-SAH) for [1-13C]acetate and [1-13C]pyruvate esters with application of PH-INEPT-type pulse sequences for 1H to 13C polarization transfer is reported, and its efficiency is compared with that of polarization transfer based on magnetic field cycling (MFC). The pulse-sequence transfer approach may have its merits in some applications because the entire hyperpolarization procedure is implemented directly in an NMR or MRI instrument, whereas MFC requires a controlled field variation at low magnetic fields. Optimization of the PH-INEPT-type transfer sequences resulted in 13C polarization values of 0.66 ± 0.04% and 0.19 ± 0.02% for allyl [1-13C]pyruvate and ethyl [1-13C]acetate, respectively, which is lower than the corresponding polarization levels obtained with MFC for 1H to 13C polarization transfer (3.95 ± 0.05% and 0.65 ± 0.05% for allyl [1-13C]pyruvate and ethyl [1-13C]acetate, respectively). Nevertheless, a significant 13C NMR signal enhancement with respect to thermal polarization allowed us to perform 13C MR imaging of both biologically relevant hyperpolarized molecules which can be used to produce useful contrast agents for the in vivo imaging applications.

Svyatova A. , Kozinenko V.P. , Chukanov N.V. , Burueva D.B. , Chekmenev E.Y. , Chen Y-W. , Hwang D.W. , Kovtunov K.V. , Koptyug I.V.
PHIP hyperpolarized [1-13C]pyruvate and [1-13C]acetate esters via PH-INEPT polarization transfer monitored by 13C NMR and MRI
Scientific Reports. 2021. V.11. N1. 5646 :1-12. DOI: 10.1038/s41598-021-85136-2 WOS Scopus OpenAlex

Поступила в редакцию:	13 сент. 2020 г.
Принята к публикации:	18 февр. 2021 г.
Опубликована в печати:	11 мар. 2021 г.

Web of science:	WOS:000629619800024
Scopus:	2-s2.0-85102478166
OpenAlex:	W3134470518

БД	Цитирований
Scopus	24
Web of science	23